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blue light transilluminator  (Clinx Science)


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    Structured Review

    Clinx Science blue light transilluminator
    Feasibility Testing. (A) Design of the LAMP primer set and crRNA using the VP1 gene sequence of the EV-A71. (B) Specific sequences of the primers and crRNA are shown, with arrows indicating the 5′ to 3′ direction. The crRNA is designed downstream of the PAM site (5′-TTTG-3′). (C) HNB-RT-LAMP colorimetric and gel electrophoresis analyses were conducted using the standalone EV-A71 primers set. (D) Multiplex HNB-RT-LAMP colorimetric and gel electrophoresis analyses were conducted using the mixed primer set for EV-A71 and universal EVs. (E) CRISPR/Cas12a detection was performed on the amplification products of the multiplex RT-LAMP using the mixed primer set for EV-A71 and EVs. Observation was made under a blue light <t>transilluminator,</t> and fluorescence intensity was analyzed using a microplate reader. The difference in fluorescence intensity between the EV-A71 group and the NTC group was statistically significant ( P < 0.001, ****), whereas other EVs showed no significant difference from the NTC ( P > 0.05, ns). The experiment was carried out three times.
    Blue Light Transilluminator, supplied by Clinx Science, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/blue+light+transilluminator/pmc11983180-52-16-20?v=Clinx+Science
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    blue light transilluminator - by Bioz Stars, 2026-08
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    Images

    1) Product Images from "HNB-RT-LAMP Coupled with CRISPR/Cas12a for the Simultaneous Detection of Enteroviruses and Enterovirus A71 in a Single Tube"

    Article Title: HNB-RT-LAMP Coupled with CRISPR/Cas12a for the Simultaneous Detection of Enteroviruses and Enterovirus A71 in a Single Tube

    Journal: ACS Omega

    doi: 10.1021/acsomega.4c09729

    Feasibility Testing. (A) Design of the LAMP primer set and crRNA using the VP1 gene sequence of the EV-A71. (B) Specific sequences of the primers and crRNA are shown, with arrows indicating the 5′ to 3′ direction. The crRNA is designed downstream of the PAM site (5′-TTTG-3′). (C) HNB-RT-LAMP colorimetric and gel electrophoresis analyses were conducted using the standalone EV-A71 primers set. (D) Multiplex HNB-RT-LAMP colorimetric and gel electrophoresis analyses were conducted using the mixed primer set for EV-A71 and universal EVs. (E) CRISPR/Cas12a detection was performed on the amplification products of the multiplex RT-LAMP using the mixed primer set for EV-A71 and EVs. Observation was made under a blue light transilluminator, and fluorescence intensity was analyzed using a microplate reader. The difference in fluorescence intensity between the EV-A71 group and the NTC group was statistically significant ( P < 0.001, ****), whereas other EVs showed no significant difference from the NTC ( P > 0.05, ns). The experiment was carried out three times.
    Figure Legend Snippet: Feasibility Testing. (A) Design of the LAMP primer set and crRNA using the VP1 gene sequence of the EV-A71. (B) Specific sequences of the primers and crRNA are shown, with arrows indicating the 5′ to 3′ direction. The crRNA is designed downstream of the PAM site (5′-TTTG-3′). (C) HNB-RT-LAMP colorimetric and gel electrophoresis analyses were conducted using the standalone EV-A71 primers set. (D) Multiplex HNB-RT-LAMP colorimetric and gel electrophoresis analyses were conducted using the mixed primer set for EV-A71 and universal EVs. (E) CRISPR/Cas12a detection was performed on the amplification products of the multiplex RT-LAMP using the mixed primer set for EV-A71 and EVs. Observation was made under a blue light transilluminator, and fluorescence intensity was analyzed using a microplate reader. The difference in fluorescence intensity between the EV-A71 group and the NTC group was statistically significant ( P < 0.001, ****), whereas other EVs showed no significant difference from the NTC ( P > 0.05, ns). The experiment was carried out three times.

    Techniques Used: Sequencing, Nucleic Acid Electrophoresis, Multiplex Assay, CRISPR, Amplification, Fluorescence



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    Feasibility Testing. (A) Design of the LAMP primer set and crRNA using the VP1 gene sequence of the EV-A71. (B) Specific sequences of the primers and crRNA are shown, with arrows indicating the 5′ to 3′ direction. The crRNA is designed downstream of the PAM site (5′-TTTG-3′). (C) HNB-RT-LAMP colorimetric and gel electrophoresis analyses were conducted using the standalone EV-A71 primers set. (D) Multiplex HNB-RT-LAMP colorimetric and gel electrophoresis analyses were conducted using the mixed primer set for EV-A71 and universal EVs. (E) CRISPR/Cas12a detection was performed on the amplification products of the multiplex RT-LAMP using the mixed primer set for EV-A71 and EVs. Observation was made under a blue light <t>transilluminator,</t> and fluorescence intensity was analyzed using a microplate reader. The difference in fluorescence intensity between the EV-A71 group and the NTC group was statistically significant ( P < 0.001, ****), whereas other EVs showed no significant difference from the NTC ( P > 0.05, ns). The experiment was carried out three times.
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    Feasibility Testing. (A) Design of the LAMP primer set and crRNA using the VP1 gene sequence of the EV-A71. (B) Specific sequences of the primers and crRNA are shown, with arrows indicating the 5′ to 3′ direction. The crRNA is designed downstream of the PAM site (5′-TTTG-3′). (C) HNB-RT-LAMP colorimetric and gel electrophoresis analyses were conducted using the standalone EV-A71 primers set. (D) Multiplex HNB-RT-LAMP colorimetric and gel electrophoresis analyses were conducted using the mixed primer set for EV-A71 and universal EVs. (E) CRISPR/Cas12a detection was performed on the amplification products of the multiplex RT-LAMP using the mixed primer set for EV-A71 and EVs. Observation was made under a blue light <t>transilluminator,</t> and fluorescence intensity was analyzed using a microplate reader. The difference in fluorescence intensity between the EV-A71 group and the NTC group was statistically significant ( P < 0.001, ****), whereas other EVs showed no significant difference from the NTC ( P > 0.05, ns). The experiment was carried out three times.
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    Feasibility Testing. (A) Design of the LAMP primer set and crRNA using the VP1 gene sequence of the EV-A71. (B) Specific sequences of the primers and crRNA are shown, with arrows indicating the 5′ to 3′ direction. The crRNA is designed downstream of the PAM site (5′-TTTG-3′). (C) HNB-RT-LAMP colorimetric and gel electrophoresis analyses were conducted using the standalone EV-A71 primers set. (D) Multiplex HNB-RT-LAMP colorimetric and gel electrophoresis analyses were conducted using the mixed primer set for EV-A71 and universal EVs. (E) CRISPR/Cas12a detection was performed on the amplification products of the multiplex RT-LAMP using the mixed primer set for EV-A71 and EVs. Observation was made under a blue light <t>transilluminator,</t> and fluorescence intensity was analyzed using a microplate reader. The difference in fluorescence intensity between the EV-A71 group and the NTC group was statistically significant ( P < 0.001, ****), whereas other EVs showed no significant difference from the NTC ( P > 0.05, ns). The experiment was carried out three times.
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    Image Search Results


    Feasibility Testing. (A) Design of the LAMP primer set and crRNA using the VP1 gene sequence of the EV-A71. (B) Specific sequences of the primers and crRNA are shown, with arrows indicating the 5′ to 3′ direction. The crRNA is designed downstream of the PAM site (5′-TTTG-3′). (C) HNB-RT-LAMP colorimetric and gel electrophoresis analyses were conducted using the standalone EV-A71 primers set. (D) Multiplex HNB-RT-LAMP colorimetric and gel electrophoresis analyses were conducted using the mixed primer set for EV-A71 and universal EVs. (E) CRISPR/Cas12a detection was performed on the amplification products of the multiplex RT-LAMP using the mixed primer set for EV-A71 and EVs. Observation was made under a blue light transilluminator, and fluorescence intensity was analyzed using a microplate reader. The difference in fluorescence intensity between the EV-A71 group and the NTC group was statistically significant ( P < 0.001, ****), whereas other EVs showed no significant difference from the NTC ( P > 0.05, ns). The experiment was carried out three times.

    Journal: ACS Omega

    Article Title: HNB-RT-LAMP Coupled with CRISPR/Cas12a for the Simultaneous Detection of Enteroviruses and Enterovirus A71 in a Single Tube

    doi: 10.1021/acsomega.4c09729

    Figure Lengend Snippet: Feasibility Testing. (A) Design of the LAMP primer set and crRNA using the VP1 gene sequence of the EV-A71. (B) Specific sequences of the primers and crRNA are shown, with arrows indicating the 5′ to 3′ direction. The crRNA is designed downstream of the PAM site (5′-TTTG-3′). (C) HNB-RT-LAMP colorimetric and gel electrophoresis analyses were conducted using the standalone EV-A71 primers set. (D) Multiplex HNB-RT-LAMP colorimetric and gel electrophoresis analyses were conducted using the mixed primer set for EV-A71 and universal EVs. (E) CRISPR/Cas12a detection was performed on the amplification products of the multiplex RT-LAMP using the mixed primer set for EV-A71 and EVs. Observation was made under a blue light transilluminator, and fluorescence intensity was analyzed using a microplate reader. The difference in fluorescence intensity between the EV-A71 group and the NTC group was statistically significant ( P < 0.001, ****), whereas other EVs showed no significant difference from the NTC ( P > 0.05, ns). The experiment was carried out three times.

    Article Snippet: Incubation was carried out at 37 °C for 10 min, followed by visual analysis under a blue light transilluminator (BlueVision200A/BV200, Clinx Science Instruments Co., Ltd.).

    Techniques: Sequencing, Nucleic Acid Electrophoresis, Multiplex Assay, CRISPR, Amplification, Fluorescence