blue light transilluminator (Clinx Science)
Structured Review

Blue Light Transilluminator, supplied by Clinx Science, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/blue+light+transilluminator/pmc11983180-52-16-20?v=Clinx+Science
Average 94 stars, based on 3 article reviews
Images
1) Product Images from "HNB-RT-LAMP Coupled with CRISPR/Cas12a for the Simultaneous Detection of Enteroviruses and Enterovirus A71 in a Single Tube"
Article Title: HNB-RT-LAMP Coupled with CRISPR/Cas12a for the Simultaneous Detection of Enteroviruses and Enterovirus A71 in a Single Tube
Journal: ACS Omega
doi: 10.1021/acsomega.4c09729
Figure Legend Snippet: Feasibility Testing. (A) Design of the LAMP primer set and crRNA using the VP1 gene sequence of the EV-A71. (B) Specific sequences of the primers and crRNA are shown, with arrows indicating the 5′ to 3′ direction. The crRNA is designed downstream of the PAM site (5′-TTTG-3′). (C) HNB-RT-LAMP colorimetric and gel electrophoresis analyses were conducted using the standalone EV-A71 primers set. (D) Multiplex HNB-RT-LAMP colorimetric and gel electrophoresis analyses were conducted using the mixed primer set for EV-A71 and universal EVs. (E) CRISPR/Cas12a detection was performed on the amplification products of the multiplex RT-LAMP using the mixed primer set for EV-A71 and EVs. Observation was made under a blue light transilluminator, and fluorescence intensity was analyzed using a microplate reader. The difference in fluorescence intensity between the EV-A71 group and the NTC group was statistically significant ( P < 0.001, ****), whereas other EVs showed no significant difference from the NTC ( P > 0.05, ns). The experiment was carried out three times.
Techniques Used: Sequencing, Nucleic Acid Electrophoresis, Multiplex Assay, CRISPR, Amplification, Fluorescence